PENGUJIAN EFEKTIVITAS EKSTRAK CAPSICUM ANNUUM SEBAGAI ANTI INFLAMASI PADA TIKUS WISTAR YANG DIINDUKSI CCL4
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Abstract
Background: Inflammation has a major role in the pathogenesis of various diseases. Non steroidal anti inflammatory drugs (NSAIDs) being the most often used drug in community, but prolonged use NSAIDs can cause digestive problems and other side effects. Capsaicin from red chilli (Capsicum annuum) extract have anti-inflammatory effects and as a replacement candidate of NSAIDs.
Methods: This research is an experimental research with pre and post test design for differential count and post test only design to leukocytes count and erythrocyte sedimentation rate (ESR). The four positive control groups and treatment group contains 5 samples of wistar rat for each group induced by CCl4 2% 0,5 ml/day for 30 days. After induction, the samples were given treatment for 14 days with ad libitum standard diet (negative control); aspirin; acetaminophen; ibuprofen; mefenamic acid (positive control), and extracts of C. annuum 2% (treatment group).
Results: The differential count from extracts of C. annuum 2% group showed a decrease in lymphocytes (p <0,001) than before treatment. While the results of leukocytes count and ESR from C. annuum extract 2% group showed smaller results, ie 5.020 ± 2.071 (p = 0,209) and 2,00 ± 1,26 (p = 0,174) compared to the NSAIDs groups.
Conclusion: Extract of C. annuum 2% were able to decrease the proportion of lymphocytes significantly, but the reduced of leukocytes count and ESR unsignificantly.
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References
Imunologi Dasar. Edisi ke-11 ed.
Jakarta: Balai Penerbit Fakultas
Kedokteran Universitas Indonesia,
2014.
2. Arifin H, Almahdy A, dan Delvita V.
Pengaruh Pemberian Vitamin C
Terhadap Fetus pada Mencit DM.
Jurnal Sains dan Teknologi Farmasi.
12:1 (2007):32-40.
3. Menichini F, Tundis R, Bonesi M,
Loizzo MR, Conforti F, et al. The
Influence of Fruit Ripening on the
Phytochemical Content and Biological Activity of Capsicum chinense
Jacq. cv Habanero. Food Chemistry.
114:1 (2009): 553-560.
4. Mueller M., Hobiger S., dan Jungbauer A. Anti-inflammatory activity ofextracts from fruits, herbs and
spices. Food Chemistry. 122:1(2010):987-996.
5. Zimmer AR, Leonardi B, Mirona D,
Schapoval E, de Oliveirac JR, et al.
Antioxidant and anti-inflammatory
pro-perties of Capsicum baccatum:
From traditional use to scientific
approach. Journal of Ethnopharmacology. 139:1 (2012): 228-233.
6. Pengurus Besar Indonesian Rheumatism Association (PB IRA).
Rekomendasi Perhimpunan Reuma
tologi Indonesia Tentang Obat Anti
Inflamasi Non Steroid. PB IRA;
2014. 22 Oktober 2016.
ekomendasi_IRA_OAINS_2014.pdf
>.
7. Meghvansi MK, Siddiqui S, Khan
MH, Gupta VK, Vairale MG, et al.
Naga Chilli: a Potential Source of
Capsaicinoids with Broadspectrum
Ethno pharmacological Applications.
Journal of Ethnopharmacology. 132:
1 (2010): 1-14.
8. Spiller F, Alves MK, Vieira SM,
Carvalho TA, Leite CE, et al. Antiinflammatory Effects of Red Pepper
(Capsicum baccatum) on Carrageenan and Antigen Induced
Inflammation. Journal of Pharmacy
and Pharmacology. 60:1 (2008):
473-478.
9. Jolayemi AT, dan Ojewole JAO.
Comparative anti inflammatory
properties of Capsaicin and ethyla
Acetate extract of Capsicum
frutescens linn (Solanaceae) in rats.
African Health Sciences. 13:2 (2013)
: 357-361.
10. Ortega MH, Moreno AO, Navarro
MDH, Cevallos GC, Alvarez LD, et
al. Antioxidant, Anti-Nociceptive, and
Anti-Inflammatory Effects of
Carotenoids Extracted from Dried
Pepper (Capsicum annuum L.).
Journal of Biomedicine and Biotechnology. (2012): 1-10.
11. Khabade VK, Lakshmeesh NB, dan
Roy S. Comparative Study on Anti
oxidant and Anti-Inflammatory
Properties of Three Colored Varieties of Capsicum annuum. Int. J. Fundamental Applied Sci. 1:3 (2012) : 51-54
12. Deepa N, Kaur C, George B, Singh
B, dan Kapoor HC. Antioxidant
Constituents in Some Sweet Pepper
(Capsicum annuum L.) Genotypes
During Maturity. Food Sci Tech; 40:1
(2007):121-129.
13. Singh UP, Suman A, Sharma M,
Singh J, Singh A, et al. HPLC
Analysis of the Phenolic Profiles in
Different Parts of Chilli (Capsicum
annum) and Okra (Abelmo-schuses
culentus L.) Moench. The Int J
Alternative Med. 5:2 (2008): 1-6.
14. López P, Gorzalczany S, Acevedo
C, Alonso R, and Ferraro G.
Chemical Study and Anti
Inflammatory activity of Capsicum
chacoense and C. baccatum.
Brazilian Journal of Pharmacognosy.
22:2 (2012): 455-458.
15. Federer WT. Procedure and
Designs Usefull for Screening
Material in Selection and Allocation
with a Bibliography. Biometrics. 19:1
(1963): 553-587.
16. Triska HCD, Lia UK, dan Kawiji.
Optimasi Ekstraksi Oleoresin Cabai
Rawit Hijau (Capsicum Fuitescens
L.) Melalui Metode Maserasi. JTP.
1:1 (2012): 58-67.
17. Yoon JH, dan Baek SJ. Molecular
targets of dietary polyphenols with
anti-inflammatory properties. Yonsei
Med. J 46:1 (2005): 585-596.
18. Kim CS, Kawada T, Kim BS, Han
IS, Choe SY, et al. Capsaicin
exhibits anti-inflammatory property
by inhibiting IkB-a degradation in
LPS-stimulated peritoneal macrophages. Cell Signal. 15:1 (2003):
299-306.
19. Liu Y, dan Nair MG. Capsaicinoids in the hottest pepper Bhut Jolokia and its antioxidant and anti inflammatory activities. Natural Product Com. 5:1 (2010): 91-94.
20. Alawi K, dan Keeble J. The paradoxical role of the transient receptor potential vanilloid 1 receptor in inflammation. Pharmacol Ther.
125:1 (2010):181-195.